Development of Anti-Idiotype Vaccine for Human Follicular Lymphoma Free Essays

string(102) " problems exist for ELISA-based detection of the tumor-specific and vaccine-induced humoral response\." Non-Hodgkin’s lymphomas (NHL) constitute a heterogeneous group of malignancies whose incidence has significantly increased in recent decades. In the year 2000, more than 145,000 cases of NHL were diagnosed in developed countries, representing thus the sixth most common cancer occurring among men and the eighth among women. Low-grade B-cell NHLs, in particular, are incurable diseases characterized by relatively slow growth and excellent initial responsiveness to chemotherapy but also by continuous relapses. We will write a custom essay sample on Development of Anti-Idiotype Vaccine for Human Follicular Lymphoma or any similar topic only for you Order Now In particular, for patients with follicular lymphoma, median overall survival (7-10 years) has not improved over the past 30 years. Although in the vast majority of patients complete or partial remissions can be obtained with either single agents or combination chemotherapy, the clinical course is characterized by a high relapse rate. After relapse, both the response rate and relapse-free survival after subsequent salvage treatment regimens steadily decrease, resulting in a median survival of only 4-5 years after the first relapse. These clinical findings, coupled with the substantial toxicities of standard treatments, have stimulated the search for novel and more tumor-selective therapies. Follicular lymphoma is a clonal B cell malignancy that expresses a unique antigen that is formed by the immunoglobulin light and heavy chains that possess highly variable regions at their amino termini. These variable regions combine to form the antigen recognition site, which can itself be recognized as an antigen, termed the idiotype. The antigen-binding site is a structural feature of each immunoglobulin that distinguishes it from other immunoglobulins. The idiotype of a particular clonal B cell lymphoma represents a tumor-specific antigen. Idiotype is a target of interest in human lymphoma. Therapeutic vaccines targeting B cell lymphoma idiotype (Id) represent a promising immunotherapeutic approach for a better clinical control of these malignancies. Immunoglobulin (Ig) molecules are composed of heavy and light chains that possess highly variable regions at their amino termini. B-cell malignancies are clonal proliferations of Ig-producing cells. The idiotypic determinants of the surface Ig can thus serve as a tumor-specific marker for the malignant clone. Indeed, both protein- and dendritic cell-based vaccines that use the patient-specific Id have resulted in clinically significant tumor-specific cellular responses with very little toxicity. A broad use of Id-based vaccination for B cell lymphomas, however, is hampered by the fact that these approaches are patient-specific so that the vaccine must be individually produced for each patient. On these grounds, new strategies obviating the need to produce customized vaccines would further simplify clinical applications of idiotypic vaccines. Goals: Goal 1: Establishment of a large database including sequences of idiotypic VH and VL genes expressed by a variety of lymphoproliferative disorders, including low grade B-NHL, autoimmunity-associated lymphoproliferations, and chronic lymphocytic leukemia. This will allow the identification of candidate Id proteins for â€Å"cross-reactive† immunotherapy. Goal 2: Pre-clinical characterization of the immunogenicity of selected natural Id proteins, with particular regard to their ability to induce immune responses against lymphoma cells expressing molecularly correlated Id proteins. The characterization will include the identification of B cell epitopes and HLA Class I-restricted cytotoxic T cell epitopes using innovative approaches and will allow the development of dedicated assays for immunomonitoring. Goal 3: Design and validation of optimized Id vaccine. Goal 4: Evaluation and validation of new adjuvants and innovative delivery systems for improved Id vaccine formulations and administration. Goal 5: â€Å"Clinical-grade† production and purification of optimized Id proteins for patient vaccination. Introduction There are approximately 65,000 new cases of non-Hodgkin’s lymphoma diagnosed each year in the US with a comparable number in Europe. Despite the use of aggressive chemotherapy and recent advances in therapy such as monoclonal antibodies (Rituxan, TM), the disease is almost invariably fatal. Follicular lymphoma (FL) patients, in particular, can have an indolent but ultimately fatal clinical course. The median relapse time for FL patients is three years, with 90% of patients dying of a tumor-related mortality within 7 years of the date of diagnosis. The clinical course is usually characterized by a series of remissions and relapses. Good response rates are seen with treatments such as chemotherapy, radiation, lymphocyte transplantation, and monoclonal antibodies. However, following initial response to treatment, the cancer invariably returns and the majority of patients relapse with resistance to all available therapy. Related B-cell derived neoplasms include multiple myeloma (approx. 15,000 cases/year in the US and chronic lymphocytic leukemia (approx. 10,000 cases/year in the US). Isolation of tumor-specific antigens (TSAs) has been a long sought-after goal for scientists involved in both basic and clinical research. Whereas tumor-associated antigens (TAAs) are localized on both normal and tumor cells, TSA are peculiar to tumor cells. This characteristic makes TSA a very desirable target for immune therapy strategies aiming to spare normal cells, or at least the indispensable ones. As regards effectors mechanisms, although some indirect evidence exists for participation of both natural killer (NK) cells (especially those activated by IL-2, known as lymphokine- activated killer, or LAK cells), and TNF-secreting macrophages in tumor immunity, most interest has been focused on the role of antigen-specific antibodies and T lymphocytes. This is particularly true among scientists developing anti-Id vaccines for human FL, even though no substantial agreement has yet been reached on which of the two main effectors pathways is most important. FL conforms to the general rule that tumors have several mechanisms to escape the attention of the immune system. The risks that Ig somatic hypermutations could result in aminoacid residue replacements leading to substantial changes within the fine immunogenic structure of the Id do not seem to be so relevant. Indeed, no such occurrence has been reported in any of the several dozen patients who have been immunized over the last decade. A much more relevant issue is the very limited ability of FL cells to present their own antigens. Although ontogenetically very close to normal mature B-lymphocytes, with respect to their normal counterparts FL cells are very poor as antigen presenting cells (APCs). This makes it rather difficult to evaluate any vaccine-induced, tumor-specific cytotoxicity even in vitro. On the other hand, no such problems exist for ELISA-based detection of the tumor-specific and vaccine-induced humoral response. You read "Development of Anti-Idiotype Vaccine for Human Follicular Lymphoma" in category "Papers" The first study of anti-Id vaccinations in humans dates only from 1992. Until then, all the work had obviously been confined to animal models. However, the accumulation of experimental data has led to the development of several promising strategies that are currently being investigated in clinical trials. These include the utilization of the Id in the form of a soluble protein or as a DNA sequence, either used to pulse dendritic cells (DCs) or else to be administered in combination with immunologic adjuvants. Soluble protein Id vaccine production is based on a hybridoma technique, which in vitro allows production of exactly the same Ig as that present on the surface of the clonal B cells of FL, or in other words the tumor-specific Id. The suspension of single cells obtained from a biopsy specimen almost invariably contains a residual population of normal B-lymphocytes alongside the tumor cells. Screening of the hybridomas by means of Ig heavy chain CDR3 PCR identification is therefore required in order to make sure that the Ig of the selected hybridoma is truly identical to the tumor- associated one.31 Once the cultured hybridoma has yielded enough purified Id, the TSA needs to be made far more immunogenic than it is in its free form. For this purpose, it may either be conjugated with a highly immunogenic carrier such as keyhole limpet hemocyanin (KLH), or else used to pulse autologous DCs. The association of a soluble protein Id vaccine with immunologic adjuvants monocyte colony-stimulating-has also proved extremely important. Granulocyte factor (GM-CSF) currently seems to be the best such adjuvant both in animal models and humans, probably because of its capacity for local recruitment of DCs in vivo at the site of vaccine injections. This step would appear to be superfluous when autologous DCs are loaded with Id ex vivo and then re-injected into the patient. A completely different alternative approach involves administration of the patients’ Id-encoding DNA sequence. With the rise of molecular technology, such DNA vaccines are beginning to come into their own. For instance, exploitation of appropriate molecular vectors (ie containing both a leader and promoter sequence) for insertion of the nucleotide sequences responsible for biosynthesis of both the Ig heavy and light chains variable regions is now relatively easy. Between the heavy- and light-chains variable regions sequences, an intertwined linker peptide must also be inserted to allow the ultimate Id-containing molecule (scFv) to fold properly. Furthermore, the vaccine can be further strengthened by adding other DNA sequences encoding for immunologic adjuvants or powerful immunogens to the vector. Finally, intramuscular injections allow progressive release of the Id following synthesis by muscular cells. In addition, this administration route seems to be associated with prolonged conservation of the genetic information within the cells without any apparent signs of integration into their genome. Experimental Design Goal 1: Establishment of a large database including sequences of idiotypic VH and VL genes expressed by a variety of lymphoproliferative disorders, including low grade B-NHL, autoimmunity-associated lymphoproliferations, and chronic lymphocytic leukemia. This will allow the identification of candidate Id proteins for â€Å"cross-reactive† immunotherapy. In establishing a database, there will be steps to follow in order to support the evidences claimed. I. Finding the cases and the occurrence of VH and VL in lymphoproliferative disorders in different hospitals and institutions that could provide valuable information for the said disorders. The facts and information should have the confirmed consent of the persons involved. II. Subjecting the cases to thorough analysis to provide the essential information needed in documenting the cases. III.   Testing the subject under the identification of Id proteins. IV. Organizing the information and establishing the database. Methods: Establishing a database to easily organize information and data needed in making the information available readily whenever they will analyze situation in which there is a suspected occurrence of lymphoproliferative disorder. By providing the information needed, they could develop system that would make things easier for them to do actions required in addressing such situation. Primary step is consolidating all the available facts and information provided that they have the consent from the owner of the information. By having the desired facts available for the reorganization of it, they could classify it according to the general category they want to use in creating their database. It could be based on severity of the case or could be base on gender or any factor that could greatly affect the situation. Then, by gathering the information they needed, the analysis of the data should be carefully done for them to eliminate excess and negligible data for an easier organization of the structure of their available resources. By implicating the main thrust of the database to the core concept of having advance cases of NHL, the higher chance they could get the information and the data based on the clinical findings of actual patients and people who suffered from that. By simply opening the way of introducing different vaccines in addressing the situation, they could develop a system of transferring and managing information that could make things easier especially in developing new technology and medicinal advancement in creating a better and more effective ways of treating such disease. By making the information more manageable, they could likely innovate an advance communication that would lead them in establishing better information and data management for the use of the development of vaccines and cures. Since they have the information but they should examine carefully every bit of information that will be a part of their set of data and facts. By looking closely to the subjects result and the specification of the action done, the development of such process in introducing a new finding on the matter should be considered. Since the goal is to establish a database that will focus on the information that could provide the facts needed on the cases diagnosed with a NHL, it is important to screen the cases as important and not negligible for them to be able to use it as a case. At the end of the process, they would go back to their primary goal and that is to establish a functional database that the core information and the key factors are integrated in a way that it would make the processing of facts and vital data would be efficient and effectively handled. Also, it will introduce technology that would compensate the fast rising of development in technological advancement even in the field of medicine. Because there are ready to use valuable information for them to handle and initialize their desired action, they would be able to commend the different opportunities in which they could get specimens and studied it for future discoveries and researches. In all, by their incorporation of the cases of NHL and their desired goal of making the information available for them to be able to easily study and review the situations and cases they previously have for them to execute and evaluate the validity of the existing tests in the current occurrence of the disease in the real place. Goal 2: Pre-clinical characterization of the immunogenicity of selected natural Id proteins, with particular regard to their ability to induce immune responses against lymphoma cells expressing molecularly correlated Id proteins. The characterization will include the identification of B cell epitopes and HLA Class I-restricted cytotoxic T cell epitopes using innovative approaches and will allow the development of dedicated assays for immunomonitoring. In dealing with the pre-classification of the immunogenicity of selected natural Id proteins, the processes involved are: I.   Accumulating soluble proteins to be tested. II. Testing them with hybridoma essential in testing the equivalence of the tumor inducing material that leads to development of the tumor. III.   Inducing the effect of the proteins and identifying its effect on B cells. IV. Using advance technique in analyzing the result and implicating with the use of the modern testing equipment and processes. Accumulation: Testing the proteins for it to be classified will be the first step. From the patients who are suffering from FL, different samples will be getting for the medical technology to be applied. Then by cultivating natural proteins, they will use it to further test the capacity of the natural cell in penetrating and deeply interacting with other Id proteins in the development of resistance to such substance. By eradicating some external factors such as the presence of other organisms, they could screen the protein level for them to be able to produce and test the Id proteins by exposing it to toxoids that could develop resistance on the desired solution. Then, the Id proteins gathered will be stored for further testing. Testing: Then, preparation of the Id protein to be tested will be carefully done in a controlled environment. Since the tumor development can not be detected by the immune system, the development of inducing material will be necessary for them to penetrate the basic defenses of the tumor. By exposing it to NKL, tumor will exhibit a different behavior but will not be extinguished. Since B cells epitope derived multiply myeloma that had been the major cause of the return of the behavior of the tumor cells, the gradual exposing it to be classified by soluble Id proteins will be dedicated. The allowance of certain percent productivity will be the basic goal of the clinical testing for them to be able to derive the pre-classification scheme that will determine substances that induce immunity on certain level with the use of soluble and Id protein present in the environment. Effect and its Identification After the testing had been carefully done, they will examine its effect on various elemental positions by trying the substance on the possible outcome. Then, FL cells will be isolated then proteins will be added to see the effect on the neoplasms produced by B-cells. Since the outcome would produce certain behavior that will exhibit a different expected one, the process will be repeatedly associated with soluble proteins to target the development of TSA since it target tumor cells. Analyzing the Result Then, the result will be analyzed in a way that it consistently produces same output. Then after looking closely and making sure that no other substance induced the effect, the validity of the result will be the next concern in analyzing the data. For it to be valuabe, the result should consist the scientific analysis of the vaccine to be introduced for them to be able to adopt a real one. Goal 3: Design and validation of optimized Id vaccine. Since the protein had been introduced in TSA that would target tumor cells, it is important to develop the next stage wherein it will pay attention to that. Tumor cells, after being extinguished by some other methods, always come back and provide a worse situation that before. It is a common problem of the development of cure because as soon as they introduce stronger antigens and antibiotics, the cells develop stronger immunity to them, making them more powerful and gave them the power to come back and come back whenever they are defeated. The result of the previous testing of the material will be used as the raw data in determining the precise development of the vaccine needed for the tumor cells. Toxoids produced by microorganisms will be introduced to the tumor cells for them to create an astounding reaction with the cells to help the antigens produce a better shield to the tumor cells. They would also address the production of its own immunity by targeting the B cells epitopes produced by the tumor cells for them to be able to weaken the effect of the tumor cells in the body. By simply having the same effect on the cells, they would establish the immunity desired. In addition, since soluble proteins produces amino acid residue, the effect of it to the development of various outgoing tumor cells will be beneficial in the sense that it would catch up the screening proves by a hard core stimulation of heavy chain CDR3 PCR. Then, the use of material that would likely predict the behavior will also introduce for the existing antigen to determine it. Goal 4: Evaluation and validation of new adjuvants and innovative delivery systems for improved Id vaccine formulations and administration. After having the result of the vaccine being tested hand-on on the tumor cells, the activation of the production of antigens will be manipulated for those to be able manage the outcome of the result. By having the systematic chain of micro toxoids that will enter the entire system of the body, they would likely produce different kinds of reactions that would benefit the production of self stimulating antigens. By exposing it to different procedures that would attest the certainty and validity of the desired production, after introducing different sets of toxoids and NK cells, the development of the self inducing multiple protein will help in eradicating the symptoms and the effect of FL. It is known that FL is fatal in terms of its effect on the human entire system. So it is important to devise a precise way of handling and dealing with it for them to be able to have an outer perspective of the natural phenomena. Id proteins will act as binders to the solutions that will be used in strengthening NK cells and TSA to promote the development and inhibit the further production of malignant development of tumor cells. By preventing the further growth of it, they would have larger revenue in which they could satisfy the needs and the improvement for having a stronger antigen. Then, natural growth of TSA will be affected by the inducing of soluble protein to target dendritic cells for them to be able to manifest the basic function of fighting foreign toxins that could affect the development of the tumor as a vital implication of the vaccine. The use of different methods in determining the feasibility of inducing the growth and the development of natural antigens that would be sufficient enough to fight the invading tumor cells will be of great use for them to be able t produce more antigens that will prevent the further worsening of the situation. Validating the use f the vaccine as one of the potential sources of defense against the foreign material invading the system would be beneficial if the could handle the needs of having a more systematized and organized level of founding a solution that would focus on the elimination of cell processes that inhibits the growth of malignant tumor like FL that is fatal to humans. Subjecting enough NK cells to further strengthening process will help them in making the process worthwhile I making a protein Id that would address the situation as founding solution to the antigen development. Goal 5: â€Å"Clinical-grade† production and purification of optimized Id proteins for patient vaccination. After developing the vaccine the process would involve the following: I.   Purifying the Vaccine to be prepared. II.   Final Verification III. Mass Production IV.   Patient Vaccination Purification After devising the vaccine, the next step is purifying it by eliminating microorganisms that would have effects on the vaccine. By continuously subjecting the vaccine into different microorganism killing environment, they would lessen the potential of having such. Radiating and constantly developing processes will be sufficient in terminating such microorganisms. Final Verification After the purification of the vaccine, a method will be done for them o be able to test if the results are really valid by having it tested for final verification. It is important to deal with it because the importance f verifying the vaccine would greatly affects its validity in the medical society. By having it tested trough lab rats or animals that have developed FL tumors; they would be injected with such vaccine for them to see if the previous results will e the same. Mass Production After the verification process, the next process will involve producing the vaccine enough for human consumption. The proteins that deal with the development of TSA would have a various report on it validity and essentialism for them to be able to have a developed system of introducing vaccines. Vaccination After the production and the vaccine is ready to use, it would be given to the patients, as long as it is approved by the medical board, to be sued as vaccine against the development of FL into malignant tumors that endangered the lives of many people. Then, by having the system of production of certain involvement of the NK cells within the hybridoma of dendritic cells, the vaccine will be of much use since it will introduce antigens that will prepare the body for the possible FL development. Since there are certain kinds of toxoids that will be introduced, there will be a harsh reaction at first to the place where it is injected because of the behavior of the toxoids and the T cells of the body. This is a sign that the vaccine is effective and doing a reaction that would strengthen the immune system of the body. Discussion After the development of the vaccine in FL, it is important to understand the need of developing such because of its adverse effect on the development of humans. It endangered the lives of many people without having the prior notification of the said disease. This is a vital step in the clinical world. Considering the existence of the natural antigens present in the environment, by the use of Id proteins that inhibits the growth of tumor cells; it would be beneficial to mankind if the continuous development will take place. By exploring the kind of the interaction ventured in this kind of process, the elemental composition of the vaccine would be developed to address the needs of the people in having the desired implication of the subject process. The processing of vaccine would include the development of stages in which it would acknowledge the presence of the cells responsible for the development of the disease. In effect, they would have a better understanding on the subject, matter and would increase the possibility of having a curable state. By implicating the notion of having a different technique in addressing the development of the vaccine, the question left for it is how long would it last for them not only to develop vaccine but also to develop a cure that would forever block the negative effects of the disease. By using and ensuring the safety of the user, they would have a proper citation of the needed plan for them to be able to execute the importance of the vaccine and its use in the modern life. Furthermore, by examining the application of the vaccine in the curing of the disease, we would see the importance of development and use of innovating techniques in determining the possible outcome of the curing of the disease. Finally, the consideration of the process if it fits the standards of the medical consideration despite the fact that there exist different processes that involve much medicinal advancement should take into consideration the impact of the introduction of this vaccine prior to the ethical understanding of the matter.   Since FL can be considered as one of the deadliest disease that one can have, the help of having a vaccine against it is beneficial to the human industry. How to cite Development of Anti-Idiotype Vaccine for Human Follicular Lymphoma, Papers

Physics lab Essay Example For Students

Physics lab Essay Buoyancy occurs because the hot air has a lower density than the cooler outside air. This lower density air weighs less than the air it replaces and that preference is the buoyancy. 2. What makes balloons float when they are full of air but not float when they are deflated? Balloons float when they are full of hot air because the hot air weighs less than the air that is in the chamber which results in a floating balloon. 3. Starting with a hot air balloon, figure out how to make the balloon float. This leaves a dot on the tape for every hit. The power source ensures that the vibration of the stylus will be regular. The period(T) of the timer it takes for the stylus to move all the way down and then up-one complete cycle. The frequency(f) of the timer is how many times the stylus goes through a cycle in a second. So the frequency. We will calculate the frequency using formula Frequency(f)=number of dots/ time, and then calculate the period using formula Period(T)= per unit time/ frequency. Procedure: 1 . Follow instructions and assemble the apparatus. . Put a carbon paper disc which is carbon-side-up on the fix shaft between the attest. 2. Place a short strip of timer tape in the recording timer over the paper disc. 3. Make sure the tape is threaded through the gates and is free of snags. 4. Turn the timer on and pull the strip through the gates. 5. Make sure you are getting dots on the underside of the tape. 6. Use the test tape to decide on the best speed to pull the tape, so that the dots are easy to read. 7. Place a +1. 5 meter piece of tape in the timer. 8. Pull the tape through a gentle, constant motion with one hand. 9. As you pull, operate the stop-watch with the other hand. The time should be close to 3 seconds. 0. Turn the ticker timer off. 11. Remove the tape and mark on it the exact time it took to run the tape. 12. Count the dots on the tape and calculate the frequency and the period of timer. 13. Re-do the above if the results are not satisfying. 14. Repeat the above for all members of the group. 15. Tabulate everyones results and compute all the results. 16. Enter all data in the table. 17. The paper tape is not straight when pulling so it may resulted in inaccurate number of dots. 3. During the experiment on two occasions the disc would either not imprint anything on to the ticker tape paper if the needle was too high or come off during the experiment as it turns. This resulted in trails having to be repeated and the waste of lab materials to retrieve accurate data. 4. The time we release and pull the tape and the operation of the stop-watch were done by two hands. This created the possibility for the tape to be released slightly before or after the timer was activated. . The ideal time is 3 seconds. However, it is next to impossible to be that exact. Such a synchronization problem can change the records of the number of dots but may not seem drastic since we are focused on the frequency and the period of the ticker timer. Percentage Error: argental error= [experiment value accepted value/ x 100 accepted value error in opened XIII Critical Analysis: This lab shows how we can further get to know a ticker timer by applying the knowledge of frequency and period. .u17ec4114b99dd911282a92b5d86066aa , .u17ec4114b99dd911282a92b5d86066aa .postImageUrl , .u17ec4114b99dd911282a92b5d86066aa .centered-text-area { min-height: 80px; position: relative; } .u17ec4114b99dd911282a92b5d86066aa , .u17ec4114b99dd911282a92b5d86066aa:hover , .u17ec4114b99dd911282a92b5d86066aa:visited , .u17ec4114b99dd911282a92b5d86066aa:active { border:0!important; } .u17ec4114b99dd911282a92b5d86066aa .clearfix:after { content: ""; display: table; clear: both; } .u17ec4114b99dd911282a92b5d86066aa { display: block; transition: background-color 250ms; webkit-transition: background-color 250ms; width: 100%; opacity: 1; transition: opacity 250ms; webkit-transition: opacity 250ms; background-color: #95A5A6; } .u17ec4114b99dd911282a92b5d86066aa:active , .u17ec4114b99dd911282a92b5d86066aa:hover { opacity: 1; transition: opacity 250ms; webkit-transition: opacity 250ms; background-color: #2C3E50; } .u17ec4114b99dd911282a92b5d86066aa .centered-text-area { width: 100%; position: relative ; } .u17ec4114b99dd911282a92b5d86066aa .ctaText { border-bottom: 0 solid #fff; color: #2980B9; font-size: 16px; font-weight: bold; margin: 0; padding: 0; text-decoration: underline; } .u17ec4114b99dd911282a92b5d86066aa .postTitle { color: #FFFFFF; font-size: 16px; font-weight: 600; margin: 0; padding: 0; width: 100%; } .u17ec4114b99dd911282a92b5d86066aa .ctaButton { background-color: #7F8C8D!important; color: #2980B9; border: none; border-radius: 3px; box-shadow: none; font-size: 14px; font-weight: bold; line-height: 26px; moz-border-radius: 3px; text-align: center; text-decoration: none; text-shadow: none; width: 80px; min-height: 80px; background: url(https://artscolumbia.org/wp-content/plugins/intelly-related-posts/assets/images/simple-arrow.png)no-repeat; position: absolute; right: 0; top: 0; } .u17ec4114b99dd911282a92b5d86066aa:hover .ctaButton { background-color: #34495E!important; } .u17ec4114b99dd911282a92b5d86066aa .centered-text { display: table; height: 80px; padding-left : 18px; top: 0; } .u17ec4114b99dd911282a92b5d86066aa .u17ec4114b99dd911282a92b5d86066aa-content { display: table-cell; margin: 0; padding: 0; padding-right: 108px; position: relative; vertical-align: middle; width: 100%; } .u17ec4114b99dd911282a92b5d86066aa:after { content: ""; display: block; clear: both; } READ: The Crucible: Although Abigail And The Girls Initi EssayThrough the lab, I knew that frequency is the number of occurrences of a repeating event per unit time and the period in seconds, meaning the number of seconds per cycle. The lab worked out well in our group with a percentage of error of less than 2% but I still found some possible improvements that can make the results more accurate: 1. We could try pulling the tape before tarring the ticker timer and the stop watch to eliminate the miscounting of the numbers of dots caused by blotching of dots at the beginning of the tape. 2. Invest in a digital photo gate to eliminate the error caused by stopping and starting the stop watch effectively. 3. We can get a more accurate results by pulling a longer tape for as so that some inevitable errors can be minimized. 4. Pull the tape along the edge of the table to have a better result of dots on the tape. Every experiment has errors, but that doesnt mean we are certain to make mistakes, we should be aware of that something we cannot control should lead to errors, yet we need to endeavor to minimize the errors by some methods. Extension Questions: 1 . Why is it important not to pull the tape too quickly? Firstly, once the tape is run out before 3 seconds, we surely need to re-do the lab, thus it would be a waste of time (shorten time for getting a more perfect result) and experiment materials. Secondly, if the dots are recorded entirely, you would find that the distance between two dots are long and harder for us to count the number of the dots. Thirdly, because of the high pulling speed, the spots on the tape maybe light- Loren, it is more likely for us to ignore some of the dots, which will certainly lead to human errors. 2. Why is it also important not to pull the tape too slowly? Pulling the tape too slowly would make the distance between two dots too short, in extreme cases, the dots may coincide, therefore it is harder for us to get exact number of the dots which furthermore will affect the accuracy of the whole experiment. 3. Does is matter whether the dots are unevenly spaced along the tape? What would that indicate? It does not matter. Since what we need is only the umber of the dots in order to calculate the frequency and the period of the ticker timer, the formula we used would average out the number of the unevenly-spaced dots during per time intervals. Therefore, the distance between any two of them doesnt affect the results. However, it indicates that we didnt pull the tape through a constant motion. 4. Would your results be more accurate if you had drawn a longer piece of tape for 5 seconds? Explain. Synchronization problem as we cannot switch on the ticker timer the same time we start the stop watch, and we cannot switch off the ticker timer the same time we stop he stop watch are inevitable. Thus however long the experiment takes, there should be some errors. Whereas, if the period and frequency are calculated by a larger number of dots and a longer experiment time, the results will be more accurate and minimize the error. In order to achieve this, we could, for example, pull a mm+ piece of tape for as. Relevant research: It is apparent that frequency is equal to the reciprocal of the period f =I/T, so it is an inverse relationship and it is a measure of the periodicity of the function. If the number of counts is not very large, it is more accurate to measure the time interval or a predetermined number of occurrences, rather than the number of occurrences within a specified time.

Marketing Cricket Communications Essay Sample free essay sample

â€Å"Unlimited talk. text. web and countrywide 3G informations. † ( Cricket Wireless. 2012 ) . Verizon. Sprint. and AT A ; T are all good known cell phone carries ; they all have been around for many old ages. Today. a new radio service has grown to the top of the charts. it is called Cricket Wireless. Even though they have merely been around 13 old ages. Cricket has expanded all over the United States. We will write a custom essay sample on Marketing Cricket Communications Essay Sample or any similar topic specifically for you Do Not WasteYour Time HIRE WRITER Only 13.90 / page This little radio company that started with selling in two provinces has now expands in over 35 provinces ( LEAP. 2012 ) . Leap Wireless International. Inc. was the name given to what is now known as Cricket Wireless. With merely 30 employees at that clip. they had a vision to supply low-cost radio service to clients all over. Leap was an international market and even though they stayed focal point on their primer market there was a new squad working on spread outing their merchandise into the United States. In 1989. Leap Cricket Wireless was born. Their first market was Chattanooga. Tennessee with an limitless service of talk for merely 30 dollars a month which was lunched March 17. 1999. In 2000. they expand into nine extra markets that conceit of Nashville. Knoxville. Memphis. Greensboro. Tulsa. Tucson. Charlotte. Small Rock and Salt Lake City. By 2002 they had thirty- four extra markets. Leap Cricket Wireless was good over one million clients. although. a twelvemonth subsequently they would register voluntary request for alleviation under chapter 11 besides known as â€Å"reorganization† bankruptcy. This merely helped their company to go what is now known as Cricket Wireless. The metempsychosis of Leap occurred in 2004 but now as Cricket Wireless. New and fresh thoughts would come to the tabular array. Their services that started with limitless talk were now spread outing to text messaging. image messaging. and long distance. By 2006. Cricket Wireless had two million clients. With few old ages in the market they had reached a larger Numberss but they still lacked in some countries. Their rivals had a larger coverage country and services like nomadic web. Cricket made this possible in 2007 ; by conveying a 3g web to their clients. They besides added a new system for payments called bridge payment ; this would let their clients to put up an extension on their measure for a period of seven yearss. The span payment would be a payment of 17 dollars per line of service and after the seven twenty-four hours period their leftover balance would be due. Cricket now included a roaming program and a larger coverage country every bit good as their web expanded into other markets like: Oklahoma. Corpus Christi. Laredo. McAllen/Brownsville. Las Vegas. St. Luis. Savannah. and Milwaukee/Madison. Expanding to these new markets allowed cricket to make to over three million clients. As engineering advanced Cricket couldn’t stay behind and in 2010. the launched their first android the Sanyo Zio by Kyocera. Cricket was offering non merely cell phone service for station paid and prepaid but they besides had internet service. There broadband was a hit with program every bit low as 35 dollar they had unlimited cyberspace for their desktop or laptop. Cricket’s new true programs that included non merely more services for less money but the 26 per centum of revenue enhancements were removed and clients now merely paid the province revenue enhancement. This increased gross revenues but the clients still demanded more. Muve Music was introduced with a program of 65 dollars a month a client non merely had over a 1000 application to take from but limitless downloads of music. This downloads were available to you in lupus erythematosuss so twenty seconds and a whole album in less than two proceedingss. Cricket celebrates the over five million clients they now p rovide service for. The start of a new twelvemonth and as summer hits Cricket launches the most expected merchandise the iphone 4g and 4s. At a starting monetary value of $ 399 and $ 499 and a rate program like no other of lone 55 and with all its services unlimited. Cricket becomes the first prepaid service to offer the iphone. The Huawie Mercury as one of Crickets best phones on the market with an 8. 0 million. pel camera and a processor of 1. 4 GHz this android gets rated in American by the PC magazine as the best in the market. Cricket now service to over six million clients countrywide and with a small over a decennary functioning they have turning and raised to the top reasonably rapidly. This little company that started with international services is now one of Americans strongest rival when it comes to wireless services. With low rate programs and low-cost devices Cricket has claimed to the top. I have worked for a cricket trade for over four old ages and when I foremost stated they had few markets and a little assortment of devices to offer. I look back and think of how much this company has grown and the sum of clients that have switched their service over to Cricket. Working with some of the Cricket reps. I have seen all the difficult work and dedication that they have put into this company as they try and delight the client. Strengths that I see in this company are like the low-cost monetary values. limitless services. and great merchandises. Failings would be the big market and the great competition against like Boost Mobile. Virgin Mobile. Sprint. Verizon. At A ; t. and many more. Opportunities they have is to make out to those who are looking for a low low-cost service and menaces that doing it so low-cost lowers the sum of money that the company brings in really twelvemonth. Never the less. Crickets has shown that they have and can remain on top while offering the best monetary values. hypertext transfer protocol: //www. leapwireless. com/who-we-are/wireless-industry

Harry Potter essays

Lord of the Rings/Harry Potter essays Although numerous critics have trashed the latest film versions of these major British works, J.K. Rowlings Harry Potter series and J.R.R. Tolkeins Hobbit and The Lord of the Rings trilogy are strong representative models of universal and classical literature, valuable to academics and laymen alike. Allusions in Harry Potter, such as the King Arthur and Excalibur legend, are modeled in the scene with the giant Basilisk fight and the triumphant sword from the Sorting Hat; King Arthur, the legend is so widely used in media, societies young and old around the world are able to identify the classical themes of strength and of power of good intentions over pervasive evil. Likewise, Homers Odyssey, being one of the oldest pieces of world literature still popular today and having entertained readers foe millennia, exhibits a basic and universal plot of the epic hero overcoming impossible odds to achieve a noble goal and this is a comparison between Odysseus epic journey through the Mediter raneans numerous mythological obstacles matched with Frodos expedition through Middle Earth returning the one Ring for Mordors destruction. The names used in Harry Potter are of Latin ancestry, such as Minerva McGonnagoll, meaning goddess Athena head of crafts, war, and wisdom, and this not only shows the allusion to the name Athena, but helps all readers understand Minervas importance in the novel. Wisdom, a major theme in both novels, common to almost every novel and a universal and classical desired trait for most communities, is implied by the head wizard, Gandolf in Lord of the Rings, meaning wise old shaman with a flowing beard and magical powers. One of the major morals taught in the novel Harry Potter is self-sacrifice, putting oneself voluntarily in a problem situation to better everyone, shown at the climax when Harry leaves his friend behind at the chess game to pursue Vol...

The Curious Case of Benjamin Button and Benjamins Character Essay

The Curious Case of Benjamin Button and Benjamins Character - Essay Example This paper stresses that Genre, Mise-en-scà ¨ne, lighting, camera angle, sound, and narrative are some of the film-making techniques used in this film. This paper is going to analyze these film-making techniques used in the film, and describe how Benjamin was able to face all the difficulties in his life. According to Campsall, a film’s genre hints what to expect in the film, and this film’s genre ranges between science fiction, romance and history. Science fiction is used because Benjamin’s condition at birth, and the way he lived from old to young cannot exist. From this paper it is clear that Romance is also a genre used in this film and it can be seen in the way Benjamin falls in love with Daisy. She developed a close friendship with him from an early age, and even though they were separated at some point, they still loved each other. The movie is set in the just after the end of the First World War, and history as a genre can be seen here. There is family who lost their child in war, and there is a time when Benjamin and a crew in a ship were in war for America. One can be able to see the livelihood of people during that time and learn a few things about history. The movie starts in a hospital, and the director only shows old Daisy lying on the bed, her daughter, and an attending nurse. Mise-en-scà ¨ne is used here because the hospital’s name is not shown, and other patients are also not shown.

Classical and Romantic Era styles in music Essay

Classical and Romantic Era styles in music - Essay Example The importance of Les Six in the 1920s of France was important not only because of the changes in classical music. There were also attempts to express the political and social changes that were occurring within France and at a global level. France was currently experiencing the political movements of revolution and war, specifically which began to emerge in the late 1800s. The time period which led to more experimentation was after World War I. The general ideology of those that were in France during this time was based on finding a sense of freedom from the past of politics with an understanding of the complexities from the suffering of war as well as the new philosophies based on freedom. This led to the exploratory nature of the music and the new expressions which showed the modern complexity of the time. The war, as well as the corruptions in politics was then able to guide in new philosophies and ideologies into a sense of modernism1. With the changes in politics, were also alte rations in identity among the French culture. This was based on the ideas of nationalism, specifically which expressed a new ideology by understanding that France needed to regain an identity outside of the wars that were being fought. The ideal of patriotism of France, as well as the identity of changing the cultural aspects that were associated with the France became the main objectives. This was combined with the concept of modernity, specifically which came from the growth of the industrial era.

Adolescent Sexual Development

Adolescent Sexual Development Kari Svendsen Abstract: This paper touches on the importance of providing public awareness and education to our educators, parents, and students so that we reduce the number of girls that have onset puberty. It also provides information on how we can teach parents to be supportive during adolescents, which can be a difficult and confusing time for students, which is more important than ever. With the improvement of sexual education programs taught in our school systems, parents and teachers can adequately prepare students for the negative effects of sexual activity before it is too late. The numbers of adolescents engaging in sexual activity, STIs and teen pregnancies can be reduced. Adolescent Sexual Development Sexual maturation and growth spurts both accompany puberty and can be a difficult and confusing time for adolescents, especially with children hitting puberty at an earlier age than ever. It is essential that adolescents have the support of their teachers, parents, and physicians at this transitional time in their lives. Even though sexual education is taught in our school systems, these students are inadequately prepared and a staggering number of students are exposed to sexually transmitted infections and teen pregnancy; not to mention the emotional distress that adolescents will endure. It takes years for the average adolescents’ body to go through the process of sexual maturation. The onset of puberty is not triggered by any particular event, but rather a multitude of things such as stress, genetics, nutrition and diet as well as the amount of body fat to name a few (Gentry, p. 1, 2002). There is also a growth spurt that is brought on by puberty when the skeletal system grows rapidly. This can occur in girls starting at age 10 and boy at age 10.5; this growth spurt usually ends around 19 for girls and 20 for boys (Headlee, p. 7, 2010). Hands legs and feet are first and then the growth spurt will hit the torso; in girls, the sexual hormone will attribute to broader hips. These growths spurts can be an awkward time for adolescent children as their bodies are evening out. Some researchers say there is no external cause for early onset puberty, while others say different. Some researchers have linked obesity as a contributing factor (Parent, p. 670, 2003). The inactivity and poor diet of our youth is putting them at risk for a higher percentage of body fat which leads to premature puberty. Environmental chemical have also been linked to an onset of puberty. Chemicals are added to almost all commercial cleaning products to include: shampoo, teething toys, bottles. Chemicals can also be in the food that adolescents eat. Another onset of puberty can be linked to the absence of a girl’s father; researchers have shown that the longer the girls has been away from her father the earlier she will start her first menstrual cycle (Parent, p. 671, 2003). Research findings show that girls who are ill-prepared for the physical and emotional changes brought on by puberty will have the most difficult time (Gentry, p. 1, 2002). This means that providing adolescents a stable and encouraging environment with family, teachers, and physicians is crucial. Beth Ross, Director of student services at Rockdale County School, expresses her concern of parent and teacher involvement after uncovering a syphilis outbreak in her school system. Rockdale County is a small, close-knit community, made up of wealthy families in a desirable part of Atlanta. Here, at least 19 females and 4 males were part of the syphilis outbreak (Ross, 1999). Through interviews and investigations it was learned that at the center of the outbreak were a group of young girls that were no older than 16 (Ross, 1999). The sexual activity would sometimes be accompanied with the use of drugs and alcohol, and the sexual interactions were with slightly older boys. These interactions would take place at one of the girl’s home when her parents were not in the house. These girls not only had sex with each other but also with all of the other boys in an open and communal environment. Beth Ross has been a counselor for over 16 years and says that the needs of children into today’s world are the same as 16 years ago when she started; they have not changed (Ross, 1999). These children have the same needs, the only thing that has changed is that both sets of parents are now working and have become too â€Å"busy† (Ross, 1999). She also points out that the young girls, who are at such shockingly young ages, that are sexually active and it stems from being asked as well as needing to feel loved and wanted. This comes from something lacking in the childhood for them to desire to become sexually active at such a young age. She mentions that these students are lacking limits and structure in their lives, lack of parents being there for them to say this is how far you go and what is acceptable. Sixteen years ago parents were more adamant about what children were and were not allowed to do so there was no question in their mind what their limits were. Some par ents say that they did not like the way they were raised and that they would never treat their child the way they were treated, but this is when the structure they hated as a child goes out of the window and where rebellion begins. Rules and structure are crucial to children. After the follow-up interview was conducted at Rockdale County, some 6-12 months later, a few of the girls were still sexually active and also still used drugs. There were just a few of the girls that said they were no longer sexually active due to the stricter rules and supervision enforced by their parents. The girls interviewed all seemed to agree that there was still a lack of communication with their parents and that no real action had been taken since the outbreak occurred. By the end of the follow-up interviews and investigation 8 of the over 20 girls were pregnant (Ross, 1999). An extensive study has been conducted by Selma N. Caal, in 2008, on high-risk sexual behavior. Caal reported that youth with highly cohesive families and low peer norms reported low risk sexual behavior; however, youth with highly cohesive families and high peer norms reported high risk sex (Caal, 2008). Youth coming from low cohesive families did not base their sexuality on their peers. If youth perceived that the peers were okay with and engaging in sexual behavior then they were more likely to be sexually active themselves (Caal, 2008). During adolescence teens try to separate from their parents and become closer to their peers, so parental communication regarding sexual behavior is crucial to the youth’s sexual activity. The more time that parents spend away from their children, the more room for risky behavior. In a time when dual parents are working full-time jobs is it harder than ever to stay in communication and enforce rules. Age also plays a factor with risky sexual behavior; as older youth were more likely for this behavior, possibly because of their sexual maturity level (Caal, 2008). Also media plays a very strong role in adolescent sexual behavior as they are exposed to it constantly, and can send messages that high-risk sexual behavior is welcomed and accepted. The age of youth engaging in sexual activity drops each year and is a great concern which proves that our youth should be better educated and monitored not only at home but also in the school system. The numbers of high-risk sexual behavior adolescents could be significantly reduced by educating our youth and holding them more accountable. Advocate for Youth reports the effectiveness of sexual education in schools has contributed to lower numbers in teen pregnancy, lower numbers of youth engaging in unprotected sex, and also delayed initiation of sexual behavior altogether (McKeon, 2006). Sexual education seems to be failing as a whole, as there are 1 million people a day who acquire a sexually transmitted infection (STI) (WHO, 2013). We are failing our youth and our world at a rate of 30 million people a month, 360 million people a year (WHO, 2013). Our adolescents account for nearly half of these numbers. STI’s are causing major infertility problems and in some cases even death. They are also costing billions of dollars annually. Unprotected sex and teen birth is still a problem; however, we are moving in the right direction as a 9 percent drop was recorded from 2009-2010 (Hamilton Ventura, 2012). This puts us at a record low rate of 34.3 births per 1000 girls between the ages of 15-19 (Hamilton Ventura, 2012). Although numbers are lowering, this is a still a major concern for both mother and child as they are rarely able to financially support themselves and cost the public 10.9 billion dollars annually (Hamilton Ventura, 2012). These staggering numbers can change if we start providing The Development Assets, created by the Search Institute, to our youth; this is a guarantee. These assets are 40 research-based encouraging qualities that affect adolescent development. By providing them to our youth, we are helping them to become caring, responsible, and productive adults. These assets have be proven to work and are quickly becoming the most positive youth development in the United States and globally. All across the world and now in other countries it is apparent the positive affect that the assets have been for all cultural and socioeconomic groups (Institute, 2007). Also, high-risk behavior is better predicted by the level of Developmental Assets one has, than their family, resources, or location. However, like with Rockdale County, most of our adolescents will have less than 40 Assets (Institute, 2007). The Developmental Assets include things such as family support, positive family communication, and service to others as well as integrity, honesty, and restraint to name a few. These assets are a stepping stone and a guideline for parents. They can especially be used in a â€Å"busy† lifestyle with both parents working full-time jobs. This checklist allows no excuses for parents who are at a loss and do not know what to do with their child. It also teaches parents and teachers how to hold the children responsible and how to discipline the children instead of allowing their actions to continue. These assets can be appropriately used from the time they are 3 years old through adolescence, and would help in never seeing situation like Rockdale County arise. The more Developmental Assets that a person is exposed to the more likely they are to thrive and be successful. Researchers show that parents can set their children up for a bright future and almost guarantee that they will not be involved in high-risk behavior such as drug and alcohol use, sexual activity and drug use, by providing more assets to their child (Institute, 2007). When our adolescents have more assets they are more diverse, have better opportunities and less likely to become involved in high-risk behaviors. There are also a few Developmental Assets that can possibly help our children to prolong their puberty. One external asset of empowerment is safety, which can be considered as the normal external world but also internal. Parents are responsible for what their children use as far as cleaning products and the food they eat, which both contain chemicals and can increase onset puberty. It is the parent’s responsibility to provide safe and desirable products and food for their children. This also leads to obesity, as it can promote onset maturation. Another external asset is constructive use of time; this means that children should participate in weekly sports club or organization within the school or community. By keeping our children active there will be less room for obesity. Another contribution to onset maturation was the absentee father, which is also covered in the assets in the support section; it states that family should provide children with a constant loving and supporting relationship. Some reasons for an absent father cannot always be avoided; however, providing the child’s father that just chooses to be absent may be swayed if he were introduced to the determent it would cause his daughter. Again, following the Developmental Assets will not only allow us to prolong puberty in girls but also allow us to provide a promising future for our children. Before researching and learning more about sexual behavior in our youth I would have said that if there were sexual education classes in our schools that they shou ld be kept to a minimum and not go into much detail on the topic, this is because of how I grew up. As a family and a school we were not open about our sexuality at all, nor did we have sexual education in our school system. And I personally, did not engage in high-risk sexual behavior nor did I know it was a concern. This could be contributed to my own Developmental Assets that my parents provided me with as there were very few, if any, that I was not given. My views have changed drastically on the need for better and more thorough sexual education programs in our schools after seeing the drastic change in numbers of teen pregnancy and STIs. We have to prevent the age of youth engaging in sexual activity dropping each year; it is a great concern and our responsibility to educate them in schools. We should provide the parents and teachers education classes addressing these issues as they may not have been educated themselves. Schools should make information more available to parents and maybe they will see the importance of this issue. Brochures that address concerns and causes for onset puberty would be beneficial to parents, and also providing information to parents at PTO meetings, and other meetings when they already have the parent’s attention. Our schools are concerned with meetings about budgets and addressing lunch programs and ignoring important issues such as sexual development. It is one thing to teach our students, but we must also teach their parents. Advocate for Youth reports the effectiveness of sexual education in schools has contributed to lower numbers in teen pregnancy, lower numbers of youth engaging in unprotected sex, and also delayed initiation of sexual behavior altogether (McKeon, 2006). I think this proves that sexual education in our schools without a doubt needed. Schools can provide sexual development information at correct ages instead of local news and radio stations trying to stop the spread of sexual activity and STIs. I know now just riding in the car with my daughter in the middle of the day listening to the radio with my daughter, there are condom commercials. She is 7 years old and should not be exposed to that for many years to come. I think the internet and television are far worse and if we are not around to monitor our children from a young age then they will be more likely to partake in these kinds of activities. School is the place to educate our children where they can ask questions and learn from adults, not just assuming on their own and among their peers. We can also offer students counseling and behavioral interventions, hopefully before, but especially after children like Rockdale County, as this would provide both parents and students to find out the underlying cause for wanting to engage in sexual activity. If counseling and treatment were more readily available student would not continue to engage in inappropriate behavior and quite possibly be prevented from becoming teen parents as we also saw with the Rockdale county students. These counseling sessions and treatments can be a more intense version of the sexual education classes in our schools that can provide students who are already sexually active and those who want to become sexually active. They can provide both pre as well as post STI test counseling so that teens can recognize symptoms of these infections, be encouraged to let their partner know, and not go untreated. Also, counseling and intervention should use condom promotion to influence students to practice safe sex and lower the risk of teen pregnancy. By providing public awareness and education to our educators, parents, and students we reduce the number of girls that have onset puberty. Parents being taught to be supportive during adolescents, which can be a difficult and confusing time for students, is more important than ever. With the improvement of sexual education programs taught in our school systems, parents and teachers can adequately prepare students for the negative effects of sexual activity before it is too late. The numbers of adolescents engaging in sexual activity, STIs and teen pregnancies can be reduced. References: Caal, Selma M. (2008) Adolescent Sexual Development: Contextualizing a Cognitive Process in the Decision to Engage in Protective or Risky Sexual Behavior. George Mason University.UMI. Web. 14 Dec. 2013. http://denhamlab.gmu.edu/Theses and Dissertations PDFs/Caal2008.pdf>. CDC. (2011). Effective HIV and STD Prevention Programs for Youth.Sexual Behaviors.http://www.cdc.gov/healthyyouth/sexualbehaviors/effective_programs.htm Gentry, J. (2002). Developing Assests. Washington: American Physcological Assosication. Hamilton, B., Ventura, S. (2012). NCHS Data Brief. Centers for Disease Control and Prevention. Retrieved December 18, 2013, from http://www.cdc.gov/nchs/data/databriefs/db89.htm Headlee, K. (2010). Growth and Development, Ages 13 to 17à ¢Ã¢â€š ¬Ã¢â‚¬ What You Need to Know. Developing Adolescents, 1, 7. Retrieved December 16, 2013, from http://lee.ifas.ufl.edu/FCS/FCSPubs/Fact_Sheets/Growth_and_Development_13-17.pdf McKeon, B. (2006). Effective Sex Education. Advocates for Youth. Retrieved December 19, 2013, from http://www.advocatesforyouth.org/publications450 Parent, A. (2003). The Timing Of Normal Puberty And The Age Limits Of Sexual Precocity: Variations Around The World, Secular Trends, And Changes After Migration. Endocrine Reviews, 24(5), 668-671. Parke, R. D. Gauvain, M. (2009).Child psychology: A contemporary viewpoint(7th ed.). Boston: McGraw-Hill. Ross, B. (1999). The Lost Children of Rockdale County. PBS. Retrieved December 21, 2013, from http://www.pbs.org/wgbh/pages/frontline/shows/georgia/interviews/beth.html Institute. (2007). 40 Developmental Assets. Search Institute. Retrieved December 12, 2013, from http://www.search-institute.org/what-we-study/developmental-assets Sexually transmitted infections (STIs). (2013). WHO. Retrieved December 20, 2013, from http://www.who.int/mediacentre/factsheets/fs110/en/